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Genes identified from RNA-seq analyses are represented as Volcano plots for 20- vs. 3.5-month-old thyroid (Fig. 2B), and 20- vs. 10-month-old thyroid (Fig. 2D). The top five upregulated and downregulated genes, as well as clock genes, are indicated by symbol-marked dots. The top five genes with the largest negative or positive standardized mean difference are represented in bar graphs (Fig. 2C, E). The genes presented in Fig. 2C, E are as follows: Arntl/Bmal1, Tekt1, Cyr61, Atf3, Egr1, Fos, and Fosb. This demonstrated significant downregulation of the circadian clock gene Arntl/Bmal1 and significant upregulation of cell proliferation, migration, and tumorigenesis genes Cyr61, Atf3, Egr1, Fos, and Fosb in thyroids of aged mice.

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